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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
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Search results for , issue "Vol 27 No 2 (2022): June 2022" : 8 Documents clear
Virtual prediction of purple rice ferulic acid as anti-inflammatory of TNF-α signaling Ernanin Dyah Wijayanti; Anna Safitri; Dian Siswanto; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20221

Abstract

Purple rice is one of the main sources of ferulic acid (FA). Some studies reported anti-inflammatory properties of FA, but the interaction of FA with TNF-α signaling has not been elucidated. TNF-α is a target for anti-inflammatory drug research due to its major role in the inflammatory process. This study aims to investigate the interaction of FA with TNF-α and TNF-α receptor (TNFR) through in silico study and evaluate the drug-like properties and biological activity of FA. The interactions among FA (CID 445858), TNF-α (2AZ5), and TNFR (1NCF) were docked by Hex 8.0.0 Cuda, then visualized by Discovery Studio 2020 and LigPlot V.1.4.5. Apigenin-7-glucuronide (AG, CID 5319484) was used as the positive control. The drug-like properties were predicted by Lipinski’s rule of five and the biological activity was analyzed by PASS online. FA showed good properties as a drug-like molecule and biological activity as an anti-inflammatory. FA also showed good interaction with TNF-α and TNFR. FA bound to TNF-α at Asn92(B), Val91(B), Leu93(B), Phe124(B), Phe124(D), and Leu93(D) residues with docking energy of -214.6 kJ/mol, and bound to TNFR at Pro16(A), Glu56(B), Cys55(B), Glu54(B) residues with docking energy of -191.1 kJ/mol. FA could inhibit TNF-α – TNFR interaction by binding to TNFR at Glu54 residue, the same inhibition mechanism to AG which bind to TNFR at Glu54 and Val90. The current study shows that FA has the potential as an anti-inflammatory of TNF-α signaling and can be developed as an oral anti-inflammatory drug candidate.
Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli Rini Purbowati; Sugiharto; Agusniar Furkani Listyawati; Masfufatun; Lusiani Tjandra; Noer Kumala Indahsari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20222

Abstract

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.
Study of soil bacteria and fungi population in oil palm with big hole planting system Rizki Desika Putri Pane; Rana Farrasati; Nuzul Hijri Darlan; Suroso Rahutomo; Heri Santoso; Eko Noviandi Ginting; Iput Pradiko; Fandi Hidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20226

Abstract

The big hole planting system for oil palm is one of the efforts to control the infection of Ganoderma boninense. The big hole method can also improve physical and chemical soil properties that affect the soil microbes population and in turn suppress the soil pathogens. However, the information about soil microbes population under the big hole planting method is still limited. This study aimed to compare the population of soil bacteria and fungi in soil samples from oil palm plantations that applied the big hole for 15 years and non big hole (standard) planting method. The population of bacteria and fungi was analyzed using the dilution plate method, while the calculation was based on the total plate count. Based on the results of statistical analysis (P-Value = 0.05), it is known that the mean population of bacteria is significantly different but the mean population of fungi is not significantly different between the big hole and non-big hole planting systems. The population of soil bacteria under the big hole and standard method was 1.6 x 106 cfu/g and 1.1 x 106 cfu/g, respectively. Meanwhile, the population of soil fungi under the big hole method was 9.7 x 104 cfu/g and 7.2 x 104 cfu/g under the standard planting method. Microorganisms in soil are considered important for maintaining soil health and quality, because various microorganisms are involved in important soil functions. In addition, high soil microbial populations will contribute to reducing soil base disease or suppression of soil pathogens.
Communities and tree-species preferences of birds in traditional agroforestry system in Meru Betiri National Park Agung Sih Kurnianto; Luhur Septiadi; Puji Firmansyah; Nilasari Dewi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20225

Abstract

Agroforestry is a critical component of providing primary habitat for birds. Meru Betiri National Park (MBNP) establishes a diverse array of ecologically and economically significant trees while allowing local residents to farm beneath them, resulting in the formation of a complex agroforestry system. The purpose of this study is to examine bird communities and their tree-species preferences across tree height levels in MBNP's traditional agroforestry system. For vegetation analysis, we used the circle method, and for bird data collection, we used the point count method in a single circle approach. For both vegetation and bird communities, the ecological indices (the important value index, Shannon-Wiener diversity index, Margalef species richness index, and evenness index) were calculated. Birds' ecological roles and activity preferences were analyzed using principal component analysis and presented in stratified tree height levels. The results indicated that insectivores’ birds are the primary insect predators in the traditional MBNP agroforestry system (11 out of 16 bird families). Artocarpus heterophyllus was the most frequently visited tree species out of seven, which may have significant ecological value (for bird diversity) and economic benefits (for avitourism). Finally, one can deduce that the majority of bird activity occurs between 4 and 20 meters above the ground.
Multiplex PCR for the detection of Salmonella spp. in Indonesian traditional shrimp paste (Terasi) Affan Gaffar; Yoga Dwi Jatmiko; Asep Awaludin Prihanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20227

Abstract

Terasi is a food condiment originating from Indonesia which is processed by fermenting shrimp, fish, or a mixture of both. The processing of terasi in Indonesia is still found traditionally so that it will affect the low quality of terasi which is characterized by being contaminated by pathogenic bacteria such as Salmonella. Salmonella is a bacterium that causes foodborne diseases. The purpose of this study was to evaluate the presence of Salmonella in shrimp paste using conventional method and then confirmed with molecular approach. This study used 10 samples consisting modern terasi and five traditional terasi. The isolation of Salmonella spp. carried out by using selective media, and then the isolates were characterized further using biochemical test (TSIA and LIA). The species identification of Salmonella spp. was carried out by multiplex PCR (mPCR). The isolates that were not detected by mPCR were further identified using 16s rDNA sequencing. The isolation results showed that Salmonella isolates were only detected in traditional terasi with a density range of 2.4 x 1052.9 x 108 CFU/g. A total of 20 isolates were characterized biochemically as Salmonella, and out of 17 isolates consistently identified as Salmonella enterica serovar Typhimurium by using mPCR. The rest three isolates were further identified as Citrobacter freundii based on 16s rDNA sequencing with similarity level of 99%. The presence of Salmonella in the shrimp paste indicates that the processing of traditional shrimp paste (terasi) should be evaluated in accordance with good manufacturing process.
Microstructure profile on selected-traditional Indonesian herbal powder Naqiyah A. Mulachelah; Syahputra Wibowo; Sri Widyarti; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20224

Abstract

Jamu is well known as traditional herbal medicine that has been used by Indonesian for centuries ago. Local people usually consume jamu for maintaining health and therapy diseases because it is cheaper and has less side effects. Scientific approach needs to be done to study the characteristic of the herbals. This research aims to characterize Indonesian herbal medicine based on microstructure using Scanning Electron Microscope (SEM). The samples for this research are rosela flower (Hibiscus sabdariffa), ginger (Zingiber officinale), lime fruit (Citrus aurantifolia), and celebes pepper (Piper crocatum). The infusion method preparation was carried out. The mixture was processed further by a freeze-drying method to obtain a powder form. SEM was utilized to capture the herbal powder image. Size of distribution and surface roughness were analysed by ImageJ and Origin Pro 8 on SEM images. The size measurement result showed that infused Rosella has the biggest grain (53.5 µm), followed by lime (43.8 µm), ginger (41.2 µm) and celebes pepper (38.4 µm). The surface roughness results showed that the roughest samples of all is ginger (Ra = 64.417), followed by lime (Ra = 58.761), celebes pepper (Ra = 43.589), and rosella (Ra = 30.855). In conclusion, based on its size measurement result, celebes pepper is the most soluble and has the highest bioavailability, followed by ginger, lime, and rosella. According to its surface roughness, celebes pepper also showed the most soluble compared to other samples, followed by lime, ginger and rosella.
Increasing antioxidant activity of soursop (Annona muricata L.) and noni (Morinda citrifolia L.) leaves fermented by Lactobacillus plantarum BP102 Dayu Nirwana Putri; Sri Widyarti; Yoga Dwi Jatmiko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20223

Abstract

Free radicals are constantly produced by either cell metabolism or from external sources. At high concentration, they induced a tissue damage called oxidative stress. Soursop leaf (Annona muricata L.) and noni leaf (Morinda citrifolia L.) are medicinal plants with potency as antioxidants. This study aimed to evaluate the capacity of Lactobacillus plantarum BP102 in elevating the antioxidant activity of soursop and noni leaves. Dried-powder and methanol extract of soursop and noni leaves were diluted with sterile distilled water 3 g/30 mL and 0.3 g/30 mL, respectively, inoculated with 1% (v/v) of L. plantarum BP102 inoculum. The antioxidant activity was carried out using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. The antioxidant activity increased in dried-powder and methanol extract of soursop and noni leaves with different activity levels after being fermented using L. plantarum BP102 based on IC50. The increasing antioxidant activity in dried-powder of soursop leaves IC50 6.41±0.06 to 0.034±0.01 mg/mL (99.5%) was higher than of the methanol extract IC50 2.78±0.00 to 0.11±0.01 mg/mL (96%). Unfortunately, the effect of fermentation towards noni leaves could only be observed in the form of methanol extract IC50 12.8±0.01 to 0.33±0.02 mg/mL (increased by 97.4%), the dried-powder of noni leaves was suspended and produced a dark color. The probiotic L. plantarum BP102 was used as a fermented agent in increasing the bioactive compounds especially related to antioxidant activity.
Three-dimension Glyceraldehyde-3-Phosphate Dehydrogenase protein structure of substitution and insertion sequences of GAPDH gene of chicken drumstick meat (Gallus gallus) Fatchiyah Fatchiyah; Rista Nikmatu Rohmah; Lidwina Faraline Tripisila; Dewi Ratih Tirto Sari; Adelia Adrianne Tapiory; Jihan Safira Ainnayah; Viona Faiqoh; Fajar Mustika Alam; Ahmad Faizal Abdul Razis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20228

Abstract

The study aimed to observed the 3-D structure of GAPDH protein and identify the GAPDH gene sequences mutation of chicken drumstick meat (Gallus gallus). The sample of chicken meat was randomly taken in four districts in Malang city. In this study, the DNA was isolated from drumstick meat chicken samples, amplified using proper primers, and then sequenced using ABI 3730xl DNA Sequencer. The DNA sequences alignments analyzed by BioEdit software and the control sequence of GAPDH gene was obtained from NCBI GenBank (sequence Gene ID: 374193). Then, the amino acid sequence and 3D structure of GAPDH protein were determined based on the change of nucleotide sequences using Swiss model and PyMol software. The nucleotide sequence of a partially GAPDH gene of drumstick meat chicken from districts two is completely different with a 97% similarity level, which found twelve nucleotides’ substitutions mutation between nucleotide base number 354 until 777 and three nucleotides inserted between T753 and G754 nucleotide base. These mutations changed the amino acid sequence and 3D structure of GAPDH protein. This result suggests that the differential drumstick chicken meat GAPDH sequences and 3D structure may induce the change of protein-protein interaction and induction.

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